Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	60,888	C→G	L107F (TTG→TTC)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	60,888	0	C	G	100.0%	15.3 / NA	8	L107F (TTG→TTC)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (1/7);  total (1/7)

AATAAGTTGATATATAAGTGACTTATTTTTTTAATAGAATTTTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTA  >  NC_007793/60827‑60978                                                              |                                                                                           aataaGTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAatacagt                                                      <  1:458287/100‑5 (MQ=255)    aaGTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATacaaagca                                                   <  1:4726706/100‑5 (MQ=255)      gTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAgcag                                                 <  1:1984075/100‑4 (MQ=255)                                          ttAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTgatgat             <  1:2166268/100‑1 (MQ=255)                                             tctCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaa          <  1:4345723/98‑3 (MQ=255)                                              ccgatctGATAACGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaag         <  1:4480747/96‑4 (MQ=255)                                                   ctGATAATGTTGAGAATATATAATATTAAGGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGtt    <  1:1037166/100‑1 (MQ=255)                                                     gatAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGttag  >  1:5250923/3‑98 (MQ=255)                                                              |                                                                                           AATAAGTTGATATATAAGTGACTTATTTTTTTAATAGAATTTTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTA  >  NC_007793/60827‑60978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑