Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,851	C→T	K53K (AAG→AAA)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,851	0	C	T	100.0%	16.8 / NA	8	K53K (AAG→AAA)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/6);  total (2/6)

GCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCA  >  NC_007793/2120793‑2120929                                                           |                                                                               cgacgataccTTGTGCTCTTAACTCTTTTTCGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTaa                                       >  1:2701041/9‑100 (MQ=255)      ttaccTTGTGCTCTTAACTCTTTTTCGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTcgc                                  >  1:5322076/4‑98 (MQ=255)                 tctTAACTCTTTTTCGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCAt                       <  1:335804/100‑1 (MQ=255)                        tCTTTTTCGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGt                <  1:1208077/100‑1 (MQ=255)                         cTTTTACGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTc               <  1:5727442/100‑1 (MQ=255)                           ttttCGAGTCTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTa             <  1:1569903/100‑1 (MQ=255)                                    cTAGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTaag    <  1:1532139/100‑2 (MQ=255)                                      aGGGTGAAATAATAACCTGTCTTTGATTGTTTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTaagct  <  1:3579797/100‑4 (MQ=255)                                                           |                                                                               GCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCA  >  NC_007793/2120793‑2120929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑