Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,100	T→A	I13F (ATT→TTT)	SAUSA300_RS10760 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,100	0	T	A	100.0%	21.2 / NA	10	I13F (ATT→TTT)	SAUSA300_RS10760	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/3);  total (7/3)

TCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTA  >  NC_007793/2120021‑2120163                                                                                |                                                                tCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCAc                                             >  1:2730768/1‑100 (MQ=255)          aaaacgacTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGt                                    >  1:1606781/3‑100 (MQ=255)          aaaacgacTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGt                                    >  1:2567724/3‑100 (MQ=255)          aaaacgacTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGt                                    >  1:5747290/3‑100 (MQ=255)          aaaacgacTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGt                                    >  1:579357/3‑100 (MQ=255)                                atgCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTcctcct              >  1:4737809/3‑100 (MQ=255)                                    tCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTCCTCCTTGtt          <  1:3770461/100‑2 (MQ=38)                                      acttCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTCCTCCTTGTcgt        <  1:2938800/98‑3 (MQ=39)                                            tAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCtt  <  1:615723/100‑2 (MQ=38)                                            tAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTCTTTTAGTCCTCCTTGTCGTCATCta  >  1:3535972/1‑100 (MQ=255)                                                                                |                                                                TCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTA  >  NC_007793/2120021‑2120163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑