Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	60,880	T→A	N110I (AAC→ATC)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	60,880	0	T	A	100.0%	13.6 / NA	7	N110I (AAC→ATC)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/7);  total (0/7)

AATAAGTTGATATATAAGTGACTTATTTTTTTAATAGAATTTTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTT  >  NC_007793/60827‑60976                                                      |                                                                                                 aataaGTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAatacagt                                                    <  1:458287/100‑5 (MQ=255)    aaGTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATacaaagca                                                 <  1:4726706/100‑5 (MQ=255)      gTTGATATATAAGTCACTTATTTTTTTAATAAAATTTTAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAgcag                                               <  1:1984075/100‑4 (MQ=255)                                          ttAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTgatgat           <  1:2166268/100‑1 (MQ=255)                                             tctCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaa        <  1:4345723/98‑3 (MQ=255)                                              ccgatctGATAACGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaag       <  1:4480747/96‑4 (MQ=255)                                                   ctGATAATGTTGAGAATATATAATATTAAGGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGtt  <  1:1037166/100‑1 (MQ=255)                                                      |                                                                                                 AATAAGTTGATATATAAGTGACTTATTTTTTTAATAGAATTTTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTT  >  NC_007793/60827‑60976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑