Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,119,944	C→T	V65I (GTT→ATT)	SAUSA300_RS10760 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,119,944	0	C	T	100.0%	38.5 / NA	16	V65I (GTT→ATT)	SAUSA300_RS10760	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/10);  total (6/10)

CTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAG  >  NC_007793/2119863‑2120031                                                                                  |                                                                                        ctcATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATc                                                                       >  1:4786458/1‑100 (MQ=255)  ctctGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCt                                                                      >  1:4229687/4‑100 (MQ=21)   cATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTa                                                                     >  1:1341256/1‑100 (MQ=255)    aTGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTaa                                                                    <  1:4007102/100‑1 (MQ=255)             cctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGccggtag                                                           <  1:4049187/100‑7 (MQ=18)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATGCTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:1746406/100‑1 (MQ=21)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCtgtacat                                                          <  1:4150486/100‑5 (MQ=21)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:1003141/100‑1 (MQ=255)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:3314481/100‑1 (MQ=255)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:4049491/100‑1 (MQ=255)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:460453/100‑1 (MQ=255)              ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                          <  1:5396183/100‑1 (MQ=255)                accATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAg                                                        >  1:5503419/1‑100 (MQ=255)                            cTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTTtcgtc                                            <  1:1247746/100‑1 (MQ=25)                              aaGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGCGCATAGATCggaagagcaca                                          >  1:5270516/1‑90 (MQ=21)                                                                      tCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTaa  >  1:3643869/1‑99 (MQ=255)                                                                                  |                                                                                        CTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAG  >  NC_007793/2119863‑2120031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑