Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,119,904	T→C	K78R (AAA→AGA)	SAUSA300_RS10760 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,119,904	0	T	C	100.0%	39.1 / NA	17	K78R (AAA→AGA)	SAUSA300_RS10760	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/11);  total (6/11)

AATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAG  >  NC_007793/2119821‑2119991                                                                                    |                                                                                        aaTAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCtgt                                                                         <  1:3283014/100‑1 (MQ=255)                         atatTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATaaat                                                 >  1:4019261/1‑99 (MQ=255)                                           ctcATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATc                               >  1:4786458/1‑100 (MQ=255)                                            ctctGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCt                              >  1:4229687/4‑100 (MQ=21)                                             cATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTa                             >  1:1341256/1‑100 (MQ=255)                                              aTGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTaa                            <  1:4007102/100‑1 (MQ=255)                                                       cctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGccggtag                   <  1:4049187/100‑7 (MQ=18)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATGCTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:1746406/100‑1 (MQ=21)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCtgtacat                  <  1:4150486/100‑5 (MQ=21)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:1003141/100‑1 (MQ=255)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:3314481/100‑1 (MQ=255)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:4049491/100‑1 (MQ=255)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:460453/100‑1 (MQ=255)                                                        ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGtat                  <  1:5396183/100‑1 (MQ=255)                                                          accATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAg                >  1:5503419/1‑100 (MQ=255)                                                                      cTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTTtcgtc    <  1:1247746/100‑1 (MQ=25)                                                                        aaGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGCGCATAGATCggaagagcaca  >  1:5270516/1‑90 (MQ=21)                                                                                    |                                                                                        AATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAG  >  NC_007793/2119821‑2119991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑