Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,119,599	C→T	intergenic (‑4/+277)	SAUSA300_RS10755 ← / ← SAUSA300_RS10760	ATPase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,119,599	0	C	T	100.0%	34.6 / NA	14	intergenic (‑4/+277)	SAUSA300_RS10755/SAUSA300_RS10760	ATPase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)

TTACCGTCAAAATTAAATTTTTCTTCTTTGATTCCGGCAAAGTTCGATATTGTTAATTTATTTATTTTCATATCCTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGTACCTCGATAATTAAATTTTTTATTCGTTCATGGCTGTC  >  NC_007793/2119525‑2119678                                                                           |                                                                                ttACCGTCAAAGTTAAATTTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCtt                                                        >  1:5226742/1‑100 (MQ=255) ttACCGTCAAAGTTAAATTTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCtt                                                        >  1:5501629/1‑100 (MQ=255) ttACCGTCAAAGTTAAATTTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCtt                                                        >  1:885311/1‑100 (MQ=255)  tACCGTCAAAGTTAAATTTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTc                                                       >  1:1403517/1‑100 (MQ=255)           agttaaattTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACct                                              <  1:4674385/98‑1 (MQ=255)              taaattTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCtt                                           <  1:1005385/100‑1 (MQ=255)              taaattTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCtt                                           <  1:2234399/100‑1 (MQ=255)              taaattTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCtt                                           <  1:5075094/100‑1 (MQ=255)                 attTTTCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAgc                                        <  1:5129016/100‑2 (MQ=255)                  tttttCTTCTTTGATTCCAGCAAAGTTCGATATAGTTAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAgcc                                       <  1:4382982/100‑3 (MQ=255)                                                     ttAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGCACCTCGATAATTAAATTTTTTATTCGTTCATGGCTg    <  1:1728571/100‑1 (MQ=255)                                                     ttAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGCACCTCGATAATTAAATTTTTTATTCGTTCATGGCTg    <  1:4113752/100‑1 (MQ=255)                                                     ttAACTTATTTATTTTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGCACCTCGATAATTAAATTTTTTATTCGTTCATGGCTg    <  1:5659911/100‑1 (MQ=255)                                                       aacttatttatttTCATATCTTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGCACCTCGATAATTAAATTTTTTATTCGTTCATGGCTGTc  >  1:432463/4‑100 (MQ=255)                                                                           |                                                                                TTACCGTCAAAATTAAATTTTTCTTCTTTGATTCCGGCAAAGTTCGATATTGTTAATTTATTTATTTTCATATCCTTCCTCATGCTCCTTTTTTAATCTTCCGATGACCTCTTAGTACCTCGATAATTAAATTTTTTATTCGTTCATGGCTGTC  >  NC_007793/2119525‑2119678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑