Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,119,185	A→C	L137L (CTT→CTG)	SAUSA300_RS10755 ←	ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,119,185	0	A	C	100.0%	73.9 / NA	29	L137L (CTT→CTG)	SAUSA300_RS10755	ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/9);  total (20/9)

TTAGCAATTTCAAACAACAAACTTCTTCGCTTCTTCCAATCTAGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATAAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGTTTCTTCGTTCGACTTCGTGAGTATTCCTTGCG  >  NC_007793/2119090‑2119277                                                                                                |                                                                                             ttAGCGATTTCAAACAACAAACTTCTTCGTTTCTTCCAATCTAGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGtt                                                                                          <  1:1541640/100‑1 (MQ=255) ggaGCGATTTCAAACAACCAACGGCTGCGTTTCTTCCAATCTAGTAAGTAAAAGGCGGGAGGATTAGGAATTAACTGGAAGAAATCTTCATCAATCAGtt                                                                                          <  1:2461128/98‑1 (MQ=255)      gaTTTCAAACAACAAACTTCTTCGTTTCTTCCAATCTAGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTtcatca                                                                                     <  1:5163225/99‑1 (MQ=255)                      tatgcgacgctcttcCAATCTAGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAAt                                                                     >  1:2192838/11‑100 (MQ=255)                                tcttcCGATCTAGTCAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACtta                                                           <  1:5665931/100‑2 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCGATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:1084067/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:1239080/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:5796450/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:552999/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:4925757/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:4664284/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:4095310/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:3704358/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:2973280/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:2876552/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:2788922/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:251085/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:23761/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:178212/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:1609222/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCATTTACTTTTATTGATtcat                                                >  1:5397840/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGATCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATtcat                                                >  1:221868/1‑100 (MQ=255)                                           aGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATAATAATCAATCAGTTCAAGAACAAGAGAAAAATAATACTTTATGTTTATTGATtcat                                                >  1:3745899/1‑100 (MQ=255)                                                   aaaTGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGata                                        >  1:4595224/1‑100 (MQ=255)                                                          tGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGtt                                 <  1:3238884/100‑1 (MQ=255)                                                           gAGGGTTCGTAATTAACTTGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGttt                                <  1:3598339/100‑1 (MQ=255)                                                                            ttGAATACATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGTTTCTTCGTTCGACTTCGTg               <  1:488650/100‑1 (MQ=255)                                                                                   cATCTTCATCAATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGTTTCTTCGTTCGACTTCGTGAGTATTc        <  1:4212100/100‑1 (MQ=255)                                                                                         catcaATCAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGTTTCTTCGTTCGACTTCGTGAGTATTCCTTGca  <  1:4273663/100‑2 (MQ=255)                                                                                                |                                                                                             TTAGCAATTTCAAACAACAAACTTCTTCGCTTCTTCCAATCTAGTAAGTTAAATGCTTGAGGGTTCGTAATTAACTTGAATACATCTTCATCAATAAGTTCATCAATACGAGCTTTATAATCCTTTACTTTTATTGATTCATCATTGATATATTGTTTCTTCGTTCGACTTCGTGAGTATTCCTTGCG  >  NC_007793/2119090‑2119277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑