Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,118,487	G→A	A370V (GCA→GTA)	SAUSA300_RS10755 ←	ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,118,487	0	G	A	95.5%	52.6 / ‑5.5	22	A370V (GCA→GTA)	SAUSA300_RS10755	ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (0/21);  total (0/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAT  >  NC_007793/2118392‑2118558                                                                                                |                                                                        tCTTGCCCTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTAcctc                                                                     <  1:3956360/100‑3 (MQ=255)   ttGCCCTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTAcctcaa                                                                   <  1:2992555/100‑5 (MQ=255)    tGCCCTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTAcctcagt                                                                  <  1:1214930/100‑6 (MQ=255)             aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTc                                                         <  1:2037124/100‑1 (MQ=255)             aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTc                                                         <  1:5236790/100‑1 (MQ=255)             aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTc                                                         <  1:5177122/100‑1 (MQ=255)             aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTc                                                         <  1:3289993/100‑1 (MQ=255)             aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATGTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTc                                                         <  1:3491524/100‑1 (MQ=255)                       attgatAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTacc                                               <  1:5716215/100‑3 (MQ=255)                         tgatAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCTCACTCACTTGTTCAGCTGGTAAcca                                             <  1:862655/100‑3 (MQ=255)                           atAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACtgtt                                           <  1:2695590/100‑1 (MQ=255)                           atAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTCCTCTTACCTCACTCACTTGTTCAGCTGGTAACtgtg                                           <  1:2418236/100‑4 (MQ=255)                                 gTTTGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACcac                                     <  1:446788/100‑1 (MQ=255)                                   ttGTATTGTTTCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACcactt                                   <  1:5787268/100‑3 (MQ=255)                                           tttCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACtaca                           <  1:1770565/100‑1 (MQ=255)                                            ttCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACtacat                          <  1:4996549/100‑1 (MQ=255)                                            ttCTAATTCTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACtacat                          <  1:163414/100‑1 (MQ=255)                                                    cTTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGGCCACAACAACTACATACATTGtc                  <  1:5750100/100‑1 (MQ=255)                                                       tCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGtcaag               <  1:2699713/100‑3 (MQ=255)                                                        cGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGtcatca              <  1:3941887/100‑1 (MQ=255)                                                        cGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGtcatca              <  1:4553018/100‑1 (MQ=255)                                                                    aTTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAt  <  1:781418/100‑1 (MQ=255)                                                                                                |                                                                        TCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAT  >  NC_007793/2118392‑2118558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑