Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	49,208	A→C	S569A (TCA→GCA)	SAUSA300_RS00210 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	49,208	0	A	C	100.0%	45.0 / NA	19	S569A (TCA→GCA)	SAUSA300_RS00210	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/10);  total (9/10)

AAAGACAATTAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCTTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGACGGTGTCCAATTTTGAAGTAACGACTTATCTTTAGTTAATCCGAGGATAGATGCAAACTCTACATCTAATTTTAGATGGT  >  NC_007793/49117‑49288                                                                                            |                                                                                 aaaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTcc                                                                          <  1:1640532/100‑1 (MQ=255) aaaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTcc                                                                          <  1:435462/100‑1 (MQ=255)  aaGACAATCGGAAATCTTCGTGAACTCCTGAAGGACCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                                                         <  1:1097196/100‑1 (MQ=255)  aaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                                                         <  1:806172/100‑1 (MQ=255)  aaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                                                         <  1:4025709/100‑1 (MQ=255)                    cGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACtt                                                       >  1:2125559/1‑100 (MQ=255)                               gAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTa                                            >  1:4003510/1‑100 (MQ=255)                                caGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTaa                                           >  1:3816400/2‑100 (MQ=255)                                     ccTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTACCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATccca                                      >  1:1975520/1‑98 (MQ=255)                                         tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt                                  <  1:1228662/100‑1 (MQ=255)                                         tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt                                  <  1:178216/100‑1 (MQ=255)                                         tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt                                  <  1:2940632/100‑1 (MQ=255)                                         tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt                                  <  1:3900929/100‑1 (MQ=255)                                          ccccTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAAGAtc                                 >  1:3578713/1‑99 (MQ=255)                                           ccctCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGATAg                                >  1:1198316/4‑100 (MQ=255)                                               cATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCGGGATAGAtcg                            >  1:5485034/1‑98 (MQ=255)                                                 ttAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGATAGATGCaa                          <  1:5496053/100‑1 (MQ=255)                                                  ttgCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGATAGATGCaaa                         >  1:1685728/3‑100 (MQ=255)                                                                         gCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGATAGATGCAAACTCTACATCTAATTTTAGATGGt  >  1:4518139/1‑100 (MQ=255)                                                                                            |                                                                                 AAAGACAATTAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCTTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGACGGTGTCCAATTTTGAAGTAACGACTTATCTTTAGTTAATCCGAGGATAGATGCAAACTCTACATCTAATTTTAGATGGT  >  NC_007793/49117‑49288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑