Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,115,967	T→C	N22S (AAT→AGT)	SAUSA300_RS10740 ←	single‑stranded DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,115,967	0	T	C	100.0%	14.2 / NA	7	N22S (AAT→AGT)	SAUSA300_RS10740	single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/6);  total (1/6)

AAGTCTGCCTCGCGCTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCATTTTGAGTGGTTCTTAATTCTGGGTCTCTAGTTAAACGACCAACTAATATTGTTCTGTTTAGCATTTATAAACCTCCAAAATAAACGGG  >  NC_007793/2115889‑2116055                                                                               |                                                                                         aaGTCTGCCTCGCGCTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTg                                                                     <  1:564185/100‑1 (MQ=255)     cTGCCTCGCGCTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTGGGtc                                                                 <  1:46246/100‑1 (MQ=255)        ccTCGCGCTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTGGGTctct                                                              <  1:2195790/100‑1 (MQ=255)            gcgcTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTGGGTCTCTAGtt                                                          <  1:4837689/100‑1 (MQ=255)             cgcTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTGGGTCTCTAGTTa                                                         <  1:2933182/100‑1 (MQ=255)                       gtgCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCACTTTGAGTGGTTCTTAATTCTGGGTCTCTAGTTAAACGACCAAc                                               >  1:3516516/1‑100 (MQ=255)                                                                    cATTTACACCACTTTGAGTGGTTCTTAATTCTGGGTCTCTAGTTAAACGACCAACTAATATTGTTCTGTTTAGCATTTATAAACCTCCAAAATAAACggg  <  1:1957987/100‑1 (MQ=255)                                                                               |                                                                                         AAGTCTGCCTCGCGCTCGCCTTGTGCATTTGTAAATGTACGGTTAACTGCTAATGTGAATGATGCTACATTTACACCATTTTGAGTGGTTCTTAATTCTGGGTCTCTAGTTAAACGACCAACTAATATTGTTCTGTTTAGCATTTATAAACCTCCAAAATAAACGGG  >  NC_007793/2115889‑2116055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑