Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,115,454	A→G	F26F (TTT→TTC)	SAUSA300_RS10735 ←	replication protein DnaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,115,454	0	A	G	100.0%	28.6 / NA	13	F26F (TTT→TTC)	SAUSA300_RS10735	replication protein DnaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/3);  total (10/3)

TTCAACGGTTACAATTTGGTTTCCTATAGGCACTTTTGCCTTTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCAAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATCAATACTTATCCAACCAGTC  >  NC_007793/2115373‑2115529                                                                                  |                                                                            ttCAACGGTTACAATTTGGTTTCCTATAGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTtctc                                                           >  1:1141681/1‑100 (MQ=255)  tCAACGGTTACAATTTGGTTTCCTATAGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTtctat                                                          <  1:5278563/100‑3 (MQ=255)                 tGGTTTCCTATAGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGcc                                           >  1:4706888/1‑100 (MQ=255)                    tttCCTATAGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAAt                                        <  1:3019053/100‑1 (MQ=255)                     ttCCTATAGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATg                                       <  1:4368965/100‑1 (MQ=255)                          ataGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGAtttt                                  >  1:4366587/1‑100 (MQ=255)                          ataGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGAtttt                                  >  1:5307713/1‑100 (MQ=255)                            aGGCACTTTTGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGa                                >  1:902138/1‑100 (MQ=255)                                    ttGCCTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCg                        >  1:2132744/1‑100 (MQ=255)                                       ccTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAATTTTGAAATTGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATc                     >  1:515227/1‑100 (MQ=255)                                       ccTCTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATc                     >  1:1185701/1‑100 (MQ=255)                                                          gctGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATCAATACTTATCCAACCAgat  >  1:3035932/3‑98 (MQ=255)                                                          cATGAGTAAATATATCCATGCTTCGAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATCAATACTTATCCAACCAGTc  >  1:1024552/1‑100 (MQ=255)                                                                                  |                                                                            TTCAACGGTTACAATTTGGTTTCCTATAGGCACTTTTGCCTTTGAATGATTCGCTTCCATGAGTAAATATATCCATGCTTCAAACTTTGAAAATGTTCTCTTTTCTTTAAATAGCCAATGATTTTGAATTGAGCGATCAATACTTATCCAACCAGTC  >  NC_007793/2115373‑2115529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑