Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,855	T→C	N226S (AAT→AGT)	SAUSA300_RS10735 ←	replication protein DnaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,855	0	T	C	100.0%	25.9 / NA	11	N226S (AAT→AGT)	SAUSA300_RS10735	replication protein DnaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/11);  total (0/11)

TGTTTAACGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCATTAAGCCATGTATTCAAAATGCTTTTAGCATATCCCCAAGTAACTTTGTTTCTGTCTTTAGCG  >  NC_007793/2114765‑2114917                                                                                           |                                                               tGTTTAACGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCagt                                                       <  1:3395038/100‑3 (MQ=255)  gTTTAACGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGt                                                      <  1:3406807/100‑1 (MQ=255)  gTTTAACGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGt                                                      <  1:4150703/100‑1 (MQ=255)       aCGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCa                                                 <  1:1451525/100‑1 (MQ=255)       aCGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCa                                                 <  1:4228244/100‑1 (MQ=255)                                 gCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTa                       <  1:3972225/100‑1 (MQ=255)                                     tCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTAAcct                   <  1:1576203/100‑3 (MQ=255)                                        aGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTAAcctagc                <  1:5753731/100‑6 (MQ=255)                                        aGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTAACCTTGt                <  1:3837462/100‑1 (MQ=255)                                                cTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTAACCTTGTTTCTGTca        <  1:4909317/100‑2 (MQ=255)                                                      aaaTGCACGTACTTGTTCAATAGATTTCAAGTTTGCACTAAGCCAAGTATTCAAAATGCTTTTAGCATATCCCCAAGTAACCTTGTTTCTGTCTTTAGCg  <  1:5627175/100‑1 (MQ=255)                                                                                           |                                                               TGTTTAACGAAAGGTTTATAATTTTGTTTTTTGCTTTCAAGTTGTTGCTTTTCAAATGCACGTACTTGTTCAATAGATTTCAAGTTTGCATTAAGCCATGTATTCAAAATGCTTTTAGCATATCCCCAAGTAACTTTGTTTCTGTCTTTAGCG  >  NC_007793/2114765‑2114917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑