Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	49,161	T→C	E584E (GAA→GAG)	SAUSA300_RS00210 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	49,161	0	T	C	100.0%	51.9 / NA	20	E584E (GAA→GAG)	SAUSA300_RS00210	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/14);  total (6/14)

CTATCTTCAATACGTCGATTATTCAGTTTGATGCTTCACAGTTGTATGATAAAGACAATTAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCTTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGACGGTGTCCAATTTTGAAGTAACGACTTATCTTTAGTTAATCCGAGGATA  >  NC_007793/49067‑49257                                                                                               |                                                                                                 cTATCTTCAATACGTCGATTATTCAGTTTGATGCTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCAtt                                                                                             <  1:1825114/100‑1 (MQ=255)      ccgatctagTCGATTATTCAGTCTGATGCTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCgg                                                                                        <  1:3597846/92‑1 (MQ=255)           tACGTCGATTATTCAGTTTGATGCTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGAttaa                                                                                   <  1:3644077/100‑3 (MQ=255)            aCGTCGATTATTCAGTTTGATGCTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTaa                                                                                  >  1:5568432/1‑100 (MQ=255)                       tCAGTTTGATGCTTCACGGTTGTATGATAAAGACAATCAGAGATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTc                                                                       >  1:4424057/1‑100 (MQ=255)                         aGTTTGATGCTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGc                                                                     <  1:4013360/100‑1 (MQ=255)                                 tcTTCACGGTTGTATGATAAAGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGt                                                             <  1:4790723/99‑1 (MQ=255)                                                   aaaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTcc                                           <  1:1640532/100‑1 (MQ=255)                                                   aaaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTcc                                           <  1:435462/100‑1 (MQ=255)                                                    aaGACAATCGGAAATCTTCGTGAACTCCTGAAGGACCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                          <  1:1097196/100‑1 (MQ=255)                                                    aaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                          <  1:806172/100‑1 (MQ=255)                                                    aaGACAATCAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCa                                          <  1:4025709/100‑1 (MQ=255)                                                                      cGTGAACTCCTGAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACtt                        >  1:2125559/1‑100 (MQ=255)                                                                                 gAAGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTa             >  1:4003510/1‑100 (MQ=255)                                                                                  caGGGCCTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTaa            >  1:3816400/2‑100 (MQ=255)                                                                                       ccTATCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTACCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATccca       >  1:1975520/1‑98 (MQ=255)                                                                                           tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt   <  1:3900929/100‑1 (MQ=255)                                                                                           tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt   <  1:2940632/100‑1 (MQ=255)                                                                                           tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt   <  1:178216/100‑1 (MQ=255)                                                                                           tCCCCTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAGGAt   <  1:1228662/100‑1 (MQ=255)                                                                                            ccccTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGCCGGTGTCCAATTTTGAATTAACGACTTATCTTTAGTTAATCCCAAGAtc  >  1:3578713/1‑99 (MQ=255)                                                                                               |                                                                                                 CTATCTTCAATACGTCGATTATTCAGTTTGATGCTTCACAGTTGTATGATAAAGACAATTAGAAATCTTCGTGAACTCCTGAAGGGCCTATCCCTTCATTAGCGGATTTAAAAAGTTCTTTCGCAGCTTTGTTATCATTTGACGGTGTCCAATTTTGAAGTAACGACTTATCTTTAGTTAATCCGAGGATA  >  NC_007793/49067‑49257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑