Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	34,179	T→G	A156A (GCT→GCG)	SAUSA300_RS00135 →	23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	34,179	0	T	G	100.0%	25.7 / NA	12	A156A (GCT→GCG)	SAUSA300_RS00135	23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/1);  total (11/1)

ATCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCTTATCATAAATGATGCGGTTT  >  NC_007793/34086‑34199                                                                                              |                     aTCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGCACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCAt                >  1:2557636/1‑100 (MQ=255)  tCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATa               <  1:3582524/100‑1 (MQ=255)    gctCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATaag             >  1:4497464/3‑99 (MQ=255)    aTTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATaag             >  1:4239812/1‑99 (MQ=255)       taGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGCGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtga          >  1:4125356/2‑100 (MQ=255)       cAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtga          >  1:2430780/1‑100 (MQ=255)       cAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtga          >  1:5737396/1‑100 (MQ=255)       cAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtga          >  1:689254/1‑100 (MQ=255)            aaaTGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtgatggta     >  1:1391197/1‑97 (MQ=255)             aaTGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtgatggtaa    >  1:4071774/1‑96 (MQ=255)              aTGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtgatggtaaa   >  1:2391225/1‑95 (MQ=255)               gtgcATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCGTATCATAAgtgatggtaaaa  >  1:4039183/4‑94 (MQ=255)                                                                                              |                     ATCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTGTTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAAGCTTATCATAAATGATGCGGTTT  >  NC_007793/34086‑34199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑