Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,476	T→A	K52N (AAA→AAT)	SAUSA300_RS10730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,476	0	T	A	100.0%	11.2 / NA	6	K52N (AAA→AAT)	SAUSA300_RS10730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/0);  total (6/0)

CCATGTAATCCCTCATATATATTCAAATAAGCTTGTTTGGTGTCCTAACTCCATTTGTTCATTATCAATAAGTGTTTTTAATTCATAATCATCTAAGTACCAACGTCGACCATTGAATTTTGTGTGTTTTAATCCAACAACTAAATGCCG  >  NC_007793/2114401‑2114550                                                                            |                                                                           ccATGTAATCCCTCATATATATTCAAATAAGCTTGTTTGGTGTCCTAACTCCATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATAc                                                    >  1:2880672/1‑100 (MQ=255)                                  tGTTTGGTGTCCTAACTCCATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATACCAACGACGACCATTAAATTTTGTATGTTTTAAt                   >  1:641807/1‑100 (MQ=255)                                               aaCTCCATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATACCAACGACGACCATTAAATTTTGTATGTTTTAATCCAACAACTAAAt      >  1:2058701/1‑100 (MQ=255)                                                   ccATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATACCAACGACGACCATTAAATTTTGTATGTTTTAATCCAACAACTAAATGCCg  >  1:2124366/1‑100 (MQ=255)                                                   ccATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATACCAACGACGACCATTAAATTTTGTATGTTTTAATCCAACAACTAAATGCCg  >  1:276911/1‑100 (MQ=255)                                                   ccATTTGTTCATTATCAATAAGTGTATTTAATTCATAATCGTCTAAATACCAACGACGACCATTAAATTTTGTATGTTTTAATCCAACAACTAAATGCCg  >  1:894983/1‑100 (MQ=255)                                                                            |                                                                           CCATGTAATCCCTCATATATATTCAAATAAGCTTGTTTGGTGTCCTAACTCCATTTGTTCATTATCAATAAGTGTTTTTAATTCATAATCATCTAAGTACCAACGTCGACCATTGAATTTTGTGTGTTTTAATCCAACAACTAAATGCCG  >  NC_007793/2114401‑2114550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑