Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,297	T→G	K36N (AAA→AAC)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,297	0	T	G	100.0%	25.0 / NA	12	K36N (AAA→AAC)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/8);  total (4/8)

GTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCGAGGCGAGCCC  >  NC_007793/2114203‑2114360                                                                                               |                                                                gTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGt                                                            <  1:976906/100‑1 (MQ=255)         aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgt                                                    <  1:3324959/100‑3 (MQ=255)         aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgg                                                    <  1:2269928/100‑3 (MQ=255)               aaCGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTTGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTAtggaa                                              <  1:2659397/100‑4 (MQ=255)                               ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                              >  1:3730944/1‑100 (MQ=255)                               ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                              >  1:3937658/1‑100 (MQ=255)                                      aTTTTCTATATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCtt                       <  1:5198868/100‑2 (MQ=255)                                      aTTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTa                       <  1:898349/100‑1 (MQ=255)                                                 ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTcc            <  1:5330478/100‑2 (MQ=255)                                                 ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCg            >  1:5139130/1‑100 (MQ=255)                                                   agcctcggCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCGAg          >  1:4870618/7‑100 (MQ=255)                                                           cATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCGAGGCGAcccc  <  1:331785/100‑1 (MQ=255)                                                                                               |                                                                GTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCGAGGCGAGCCC  >  NC_007793/2114203‑2114360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑