Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,290	C→T	E39K (GAA→AAA)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,290	0	C	T	100.0%	35.9 / NA	14	E39K (GAA→AAA)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/10);  total (4/10)

ATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCGAGGCGAGCCC  >  NC_007793/2114197‑2114360                                                                                              |                                                                       gttcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtata                                                                  <  1:2983994/96‑1 (MQ=255)   tcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtatagg                                                                <  1:4005969/98‑3 (MQ=255)       gTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGt                                                            <  1:976906/100‑1 (MQ=255)               aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgt                                                    <  1:3324959/100‑3 (MQ=255)               aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgg                                                    <  1:2269928/100‑3 (MQ=255)                     aaCGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTTGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTAtggaa                                              <  1:2659397/100‑4 (MQ=255)                                     ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                              >  1:3730944/1‑100 (MQ=255)                                     ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                              >  1:3937658/1‑100 (MQ=255)                                            aTTTTCTATATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCtt                       <  1:5198868/100‑2 (MQ=255)                                            aTTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTa                       <  1:898349/100‑1 (MQ=255)                                                       ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTcc            <  1:5330478/100‑2 (MQ=255)                                                       ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCg            >  1:5139130/1‑100 (MQ=255)                                                         agcctcggCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCGAg          >  1:4870618/7‑100 (MQ=255)                                                                 cATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCGAGGCGAcccc  <  1:331785/100‑1 (MQ=255)                                                                                              |                                                                       ATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCGAGGCGAGCCC  >  NC_007793/2114197‑2114360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑