Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,256	T→C	K50R (AAG→AGG)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,256	0	T	C	100.0%	27.7 / NA	12	K50R (AAG→AGG)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/9);  total (3/9)

ATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCG  >  NC_007793/2114197‑2114350                                                            |                                                                                               gttcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtata                                                        <  1:2983994/96‑1 (MQ=255)   tcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtatagg                                                      <  1:4005969/98‑3 (MQ=255)       gTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGt                                                  <  1:976906/100‑1 (MQ=255)               aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgt                                          <  1:3324959/100‑3 (MQ=255)               aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgg                                          <  1:2269928/100‑3 (MQ=255)                     aaCGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTTGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTAtggaa                                    <  1:2659397/100‑4 (MQ=255)                                     ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                    >  1:3730944/1‑100 (MQ=255)                                     ttAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCttt                    >  1:3937658/1‑100 (MQ=255)                                            aTTTTCTATATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCtt             <  1:5198868/100‑2 (MQ=255)                                            aTTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTa             <  1:898349/100‑1 (MQ=255)                                                       ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTcc  <  1:5330478/100‑2 (MQ=255)                                                       ttAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTATGTGTGTACGTATCTGCCTTTTGTGCTAAAACGCGGTCg  >  1:5139130/1‑100 (MQ=255)                                                            |                                                                                               ATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGTGTGCATATCTACCTTTTGTGCTAAAACGCGGTCG  >  NC_007793/2114197‑2114350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑