Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,228	A→G	I59I (ATT→ATC)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,228	0	A	G	100.0%	14.8 / NA	8	I59I (ATT→ATC)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/8);  total (0/8)

AGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGT  >  NC_007793/2114147‑2114316                                                                                  |                                                                                         aGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGATTCAGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCt                                                                        <  1:2463673/100‑1 (MQ=255)               gTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGATTCAGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGcctgta                                                          <  1:5760211/100‑6 (MQ=255)                                                   gttcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtata                      <  1:2983994/96‑1 (MQ=255)                                                     tcaGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGtatagg                    <  1:4005969/98‑3 (MQ=255)                                                         gTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGt                <  1:976906/100‑1 (MQ=255)                                                                 aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgt        <  1:3324959/100‑3 (MQ=255)                                                                 aagtaaaACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTGGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCatgg        <  1:2269928/100‑3 (MQ=255)                                                                       aaCGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGCCTTTGCATTTGACTTTGTAAATATTTTTTATGTTTTGTATAGTTTGTAGGCATGTAtggaa  <  1:2659397/100‑4 (MQ=255)                                                                                  |                                                                                         AGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGGCATTTGATTTTGTAAATATTTTTTATGTTCTGTATATTTTGTAGGCATATATGTGT  >  NC_007793/2114147‑2114316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑