Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,176	T→C	T77A (ACT→GCT)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,176	0	T	C	100.0%	22.9 / NA	10	T77A (ACT→GCT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/10);  total (0/10)

AAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGG  >  NC_007793/2114091‑2114260                                                                                      |                                                                                     acatggccattACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCg                                                                        <  1:5410190/91‑1 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:1165483/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:1778348/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:272842/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:4125963/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:4263033/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:4299599/100‑2 (MQ=255)           tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                              <  1:5475324/100‑2 (MQ=255)                                                         aGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGATTCAGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCt                <  1:2463673/100‑1 (MQ=255)                                                                       gTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGATTCAGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCTAGATTTAGcctgta  <  1:5760211/100‑6 (MQ=255)                                                                                      |                                                                                     AAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCTAGATTTAGCTTTGG  >  NC_007793/2114091‑2114260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑