Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,150	T→C	V85V (GTA→GTG)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,150	0	T	C	100.0%	34.7 / NA	14	V85V (GTA→GTG)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/12);  total (2/12)

AGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCT  >  NC_007793/2114057‑2114246                                                                                              |                                                                                                 aGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACtttt                                                                                            >  1:1250598/1‑100 (MQ=255)         cTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTcc                                                                                    <  1:2261277/100‑1 (MQ=255)          tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa                                                                                   <  1:2027841/100‑1 (MQ=255)          tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa                                                                                   <  1:2618373/100‑1 (MQ=255)           gTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCaa                                                                                  >  1:4603679/1‑100 (MQ=255)                                   acatggccattACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCg                                                          <  1:5410190/91‑1 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:1165483/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:1778348/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:272842/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:4125963/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:4263033/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:4299599/100‑2 (MQ=255)                                             tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc                                                <  1:5475324/100‑2 (MQ=255)                                                                                           aGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGATTCAGGTGGAGTAAAGTAAAACGCTAATTCGATTTTTAATGCATTTTCt  <  1:2463673/100‑1 (MQ=255)                                                                                              |                                                                                                 AGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTTAGGTGGAGTAAAGTAAAACTCTAATTCAATTTTTAATGCATTTTCT  >  NC_007793/2114057‑2114246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑