Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,108	G→A	D99D (GAC→GAT)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,108	0	G	A	100.0%	42.8 / NA	16	D99D (GAC→GAT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/13);  total (3/13)

GCTTTGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTT  >  NC_007793/2114047‑2114200                                                              |                                                                                             gatctgggCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGctt                                                        <  1:5668041/93‑1 (MQ=255)    ttGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCggctcaga                                                     >  1:1749587/1‑94 (MQ=255)    ttGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCggccagta                                                     <  1:707091/100‑7 (MQ=255)           aGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACtttt                                              >  1:1250598/1‑100 (MQ=255)                   cTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTcc                                      <  1:2261277/100‑1 (MQ=255)                    tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa                                     <  1:2027841/100‑1 (MQ=255)                    tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa                                     <  1:2618373/100‑1 (MQ=255)                     gTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCaa                                    >  1:4603679/1‑100 (MQ=255)                                             acatggccattACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCg            <  1:5410190/91‑1 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:1165483/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:1778348/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:272842/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:4125963/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:4263033/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:4299599/100‑2 (MQ=255)                                                       tACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCAATCGCTTGAGCTTTCTTTTTCTTCGACCATGAttc  <  1:5475324/100‑2 (MQ=255)                                                              |                                                                                             GCTTTGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAATCGCTTGAGTTTTCTTTTTCTTCGACCATGATTT  >  NC_007793/2114047‑2114200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑