Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,691	T→A	P99P (CCA→CCT)	SAUSA300_RS10720 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,691	0	T	A	100.0%	21.0 / NA	9	P99P (CCA→CCT)	SAUSA300_RS10720	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/8);  total (1/8)

TGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCATCAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGTGGTACATTAAACAAATGTGGCTTCTTTCTTCTTAGCTCAGCCTCTTTCTTTCGCTTTCTTTCCAATTTGCGTTCGAGTCTAGCTTGTT  >  NC_007793/2113597‑2113779                                                                                               |                                                                                         tgcgtgcgTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCAACAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTAc                                                                                     <  1:944275/100‑1 (MQ=255)            tCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCAACAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACaagt                                                                          <  1:2480259/100‑3 (MQ=255)                                 aCTTTCACGAATATGTCGTTTTCCAACAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAggt                                                     <  1:3430353/100‑3 (MQ=255)                                                       ccAACAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGctttct                               <  1:5037240/100‑1 (MQ=255)                                                         aacAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGctttcttt                             >  1:659089/3‑100 (MQ=255)                                                           cAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGCTTTCTTTcc                           <  1:2730774/100‑1 (MQ=255)                                                                       aTAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGCTTTCTTTCCAATTTGCGttgg               <  1:556922/100‑3 (MQ=255)                                                                        tAACGTCCTCTTGGATGTTTCTGAGGTACATTAAACAAGTGTGACTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGCTTTCTTTCCAATTTGCGTTCgt              <  1:1694293/100‑2 (MQ=255)                                                                                    ggATGTTTCTGAGGTACATTAAACAAGTGTGGCTTCTTTCTTCTTAGCTCAGCCTCTCTCTTTCGCTTTCTTTCCAATTTGCGTTCGAGTCTAGCTTGtt  <  1:2845400/100‑1 (MQ=255)                                                                                               |                                                                                         TGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCATCAGGTAGCACGCATAACGTCCTCTTGGATGTTTCTGTGGTACATTAAACAAATGTGGCTTCTTTCTTCTTAGCTCAGCCTCTTTCTTTCGCTTTCTTTCCAATTTGCGTTCGAGTCTAGCTTGTT  >  NC_007793/2113597‑2113779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑