Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,569	G→A	S16F (TCT→TTT)	SAUSA300_RS10715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,569	0	G	A	100.0%	11.4 / NA	6	S16F (TCT→TTT)	SAUSA300_RS10715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

AAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCATCAGGTAGCA  >  NC_007793/2113507‑2113663                                                               |                                                                                               taGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAAATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGtt                                                           >  1:2413561/2‑100 (MQ=255)   gTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAAATCCAAAGAATTGATTTAGGTATTCTTTgcgtgcgtggc                                                         <  1:3810045/100‑4 (MQ=255)               acATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAAATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCt                                             >  1:2401835/1‑100 (MQ=255)                                           ccTGATACAGATATCTCTTAAATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGtg                 <  1:290105/100‑2 (MQ=255)                                            cTGATACAGATATCTCTTAAATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGGAGttt                <  1:574831/100‑1 (MQ=255)                                                          tctTAAATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCAACAGGTAGCa  <  1:4229616/100‑1 (MQ=255)                                                               |                                                                                               AAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATCTACTTCTTAACTTTCACGAATATGTCGTTTTCCATCAGGTAGCA  >  NC_007793/2113507‑2113663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑