Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	48,929	G→A	intergenic (‑38/+196)	SAUSA300_RS00205 ← / ← SAUSA300_RS00210	recombinase RecA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	48,929	0	G	A	100.0%	15.1 / NA	7	intergenic (‑38/+196)	SAUSA300_RS00205/SAUSA300_RS00210	recombinase RecA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

GCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATGACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATGGATGAATTGATATATAAGCCCAACATCAATGAGATGTTGGGCGTCCATATTAGTCATTTGTTTGATTGATTTCTTC  >  NC_007793/48833‑49005                                                                                                 |                                                                             gctgcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGAt                                                                           >  1:1436144/1‑100 (MQ=255) cctgcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGAt                                                                           >  1:4027447/2‑100 (MQ=255)    gcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGATGaa                                                                        >  1:3251661/1‑100 (MQ=255)                             tttGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGATGAATTGATATATAAGCCCAACATCAATg                                               <  1:5281017/100‑1 (MQ=255)                                               taaCTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGAGGAATTGATATATAAGCCCAACATCAATGAGATGTTGGGCGTCCata                             >  1:3295613/3‑100 (MQ=255)                                                         cATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGATGAATTGATATATAAGCCCAACATCAATGAGATGTTGGGCGTCCATATTAGTCAttt                   <  1:2134484/100‑1 (MQ=255)                                                                          tCACAGTGATAATATATATTTATAGATGAATTGATATATAAGCCCAACATCAATGAGATGTTGGGCGTCCATATTAGTCATTCGTTTGATTGATttcttc  >  1:1284511/1‑100 (MQ=255)                                                                                                 |                                                                             GCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATGACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATGGATGAATTGATATATAAGCCCAACATCAATGAGATGTTGGGCGTCCATATTAGTCATTTGTTTGATTGATTTCTTC  >  NC_007793/48833‑49005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑