Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,266	G→A	D33D (GAC→GAT)	SAUSA300_RS10710 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,266	0	G	A	100.0%	28.0 / NA	11	D33D (GAC→GAT)	SAUSA300_RS10710	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/10);  total (1/10)

TACGCTCTCTTAACTT‑AGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAATCCTGTTTCATAGTTCACTTTC  >  NC_007793/2113229‑2113362                                       |                                                                                                 gcttttctctTAACTTCCGCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTCTTCTCATCTTCTTCAACCTAGCGTCCAttt                                     <  1:2430125/95‑2 (MQ=255)              cttccgatctatCCCTAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGag                        <  1:2342508/91‑2 (MQ=255)                    ccatatCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGt                  <  1:4945943/98‑1 (MQ=255)                    atctatCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTgc                  <  1:1842290/97‑2 (MQ=255)                        atCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCa              >  1:2388463/1‑100 (MQ=255)                         tCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:3838773/100‑1 (MQ=255)                         tCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:4481979/100‑1 (MQ=255)                         tCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:741866/100‑1 (MQ=255)                                   gCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGTTCattct   <  1:4452013/100‑5 (MQ=255)                                   gCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGTTCagtct   <  1:4452004/100‑5 (MQ=255)                                    cTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGTTCattctt  <  1:5557544/100‑6 (MQ=255)                                       |                                                                                                 TACGCTCTCTTAACTT‑AGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAATCCTGTTTCATAGTTCACTTTC  >  NC_007793/2113229‑2113362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑