Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,002	T→A	T121T (ACA→ACT)	SAUSA300_RS10710 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,002	0	T	A	100.0%	19.5 / NA	10	T121T (ACA→ACT)	SAUSA300_RS10710	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/4);  total (6/4)

ACGTCTTCAATCTCTTTTAGCAATCCCTCTGTGTCATTGCCGTTATACGCGCTAGCACTGATAACTGATTGTTCGATTTGTTCGCGGTTATTCATTTGTGTCATCCTCCATTTGCCCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCATTCGGCACAATATAACACTTAAGT  >  NC_007793/2112906‑2113087                                                                                                 |                                                                                      gctcttccgaTCTTTTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTagtg                                                                                    <  1:3264807/91‑1 (MQ=11) acGTCCTCAATCTCTTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTagtg                                                                                    >  1:1203415/1‑100 (MQ=11)            ctctTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCAt                                                                         >  1:1586170/1‑100 (MQ=14)             tgctcttccgaTCTCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCAtt                                                                        <  1:4082665/90‑1 (MQ=9)                         cccTCTGTATCATTCCTGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAAt                                                            >  1:5412142/1‑100 (MQ=14)                           ctctGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTc                                                          >  1:653575/1‑100 (MQ=17)                               gtatCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAg                                                      <  1:5093102/97‑1 (MQ=17)                                                          cttaTAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTcc                           <  1:3858899/97‑1 (MQ=37)                                                           ttaTAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCa                          <  1:4433875/98‑1 (MQ=37)                                                                                cttCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCATTCGGCACAATATAACACTTa     >  1:3379769/2‑100 (MQ=255)                                                                                 ttCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCATTCGGCACAATATAACACTTaa    >  1:4536803/1‑100 (MQ=255)                                                                                   cgcgGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCATTCGGCACAATATAACACTTAAGt  >  1:601387/1‑100 (MQ=255)                                                                                                 |                                                                                      ACGTCTTCAATCTCTTTTAGCAATCCCTCTGTGTCATTGCCGTTATACGCGCTAGCACTGATAACTGATTGTTCGATTTGTTCGCGGTTATTCATTTGTGTCATCCTCCATTTGCCCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCATTCGGCACAATATAACACTTAAGT  >  NC_007793/2112906‑2113087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑