Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,112,971	T→G	S10S (TCA→TCC)	SAUSA300_RS10705 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,112,971	0	T	G	100.0%	12.7 / NA	9	S10S (TCA→TCC)	SAUSA300_RS10705	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/5);  total (4/5)

ACGTCTTCAATCTCTTTTAGCAATCCCTCTGTGTCATTGCCGTTATACGCGCTAGCACTGATAACTGATTGTTCGATTTGTTCGCGGTTATTCATTTGTGTCATCCTCCATTTGCCCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCA  >  NC_007793/2112906‑2113063                                                                  |                                                                                             gctcttccgaTCTTTTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTagtg                                                            <  1:3264807/91‑1 (MQ=11) acGTCCTCAATCTCTTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTagtg                                                            >  1:1203415/1‑100 (MQ=11)            ctctTTTAGCAATCCCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCAt                                                 >  1:1586170/1‑100 (MQ=14)             tgctcttccgaTCTCTCTGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCAtt                                                <  1:4082665/90‑1 (MQ=9)                         cccTCTGTATCATTCCTGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAAt                                    >  1:5412142/1‑100 (MQ=14)                           ctctGTATCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTc                                  >  1:653575/1‑100 (MQ=17)                               gtatCATTCCCGTTATACGCACTAGCACTTATAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAg                              <  1:5093102/97‑1 (MQ=17)                                                          cttaTAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTcc   <  1:3858899/97‑1 (MQ=37)                                                           ttaTAACGGACTGTTCAATTTGTTCGCGGTTATTCATTAGTGTCTTCCTCCATTTGATCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCa  <  1:4433875/98‑1 (MQ=37)                                                                  |                                                                                             ACGTCTTCAATCTCTTTTAGCAATCCCTCTGTGTCATTGCCGTTATACGCGCTAGCACTGATAACTGATTGTTCGATTTGTTCGCGGTTATTCATTTGTGTCATCCTCCATTTGCCCTAAAAATTCGTAGAACTCATTTGTTCCGTCTAGTTTGTCCA  >  NC_007793/2112906‑2113063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑