Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	48,880	G→A	V4V (GTC→GTT)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	48,880	0	G	A	100.0%	55.6 / NA	21	V4V (GTC→GTT)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/17);  total (4/17)

TGAATATGATGTTTTTCGGCTATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATGACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATGGATGAATTGATATATAAGCCCAACATCAATG  >  NC_007793/48788‑48960                                                                                             |                                                                                 tGAATATGATGTTTTTCGGCTATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGta                                                                           <  1:514906/100‑2 (MQ=255)   aaTATGATGTTTTTCGGCTATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTc                                                                         <  1:3765415/100‑1 (MQ=255)                     tATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTc                                                       <  1:193314/100‑1 (MQ=255)                     tATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTc                                                       <  1:5585344/100‑1 (MQ=255)                       ttGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTcac                                                     <  1:2358628/100‑1 (MQ=255)                         gCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAg                                                   <  1:3857588/100‑1 (MQ=255)                          cTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAgc                                                  <  1:3245552/100‑2 (MQ=255)                          cTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAga                                                  <  1:908076/100‑2 (MQ=255)                           ttCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAgcg                                                 <  1:4534206/100‑3 (MQ=255)                            tCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGa                                                <  1:675088/100‑1 (MQ=255)                             cGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGat                                               <  1:3224009/100‑1 (MQ=255)                                    ttGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAatatat                                        <  1:3845871/100‑1 (MQ=255)                                       tttCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTTATAATATATAtt                                     <  1:403276/100‑1 (MQ=255)                                        ttCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATAttt                                    <  1:5505469/100‑1 (MQ=255)                                          cTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTAt                                  <  1:74707/100‑1 (MQ=255)                                            gtgCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCGCCTCCTTTCACAGTGATAATATATATTTAtag                                <  1:2540216/100‑3 (MQ=255)                                            gtgCTGCAAGAGACTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAagatcggaagagca                                >  1:5729820/1‑90 (MQ=255)                                              gctgcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGAt                              >  1:1436144/1‑100 (MQ=255)                                              cctgcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGAt                              >  1:4027447/2‑100 (MQ=255)                                                 gcAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGATGaa                           >  1:3251661/1‑100 (MQ=255)                                                                          tttGACGTAAATAGCCTATAACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATAGATGAATTGATATATAAGCCCAACATCAATg  <  1:5281017/100‑1 (MQ=255)                                                                                             |                                                                                 TGAATATGATGTTTTTCGGCTATTGCTTCGATAGCTTGTTTCTGTGCTGCAAGAGATTGTTGTTTCATCGTACTTTGACGTAAATAGCCTATGACTTGTTTCATATCGGCTCCTCCTTTCACAGTGATAATATATATTTATGGATGAATTGATATATAAGCCCAACATCAATG  >  NC_007793/48788‑48960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑