Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,110,154	G→T	intergenic (‑38/+194)	SAUSA300_RS10660 ← / ← SAUSA300_RS10665	HNH endonuclease/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,110,154	0	G	T	100.0%	11.6 / NA	6	intergenic (‑38/+194)	SAUSA300_RS10660/SAUSA300_RS10665	HNH endonuclease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/3);  total (3/3)

TGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGC  >  NC_007793/2110060‑2110245                                                                                               |                                                                                            tgttgtTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACTCATCt                                                                                        <  1:4782094/100‑1 (MQ=255)    tgttTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACTCATCtatg                                                                                     <  1:5620002/100‑2 (MQ=255)                  tAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACTCATCTATACTTGATGTgtagta                                                                       >  1:2676639/1‑100 (MQ=255)                                                      tcatAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACTCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATagcac                                   <  1:3434877/100‑5 (MQ=255)                                                                      tatataACTTAAGTAGTATCAAGACTCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGacac                   >  1:4071493/1‑100 (MQ=255)                                                                                       cgcAAGACTCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATgcgc  >  1:1772508/3‑100 (MQ=255)                                                                                               |                                                                                            TGTTGTTTGCCATTCTTTAGACTTATAGAATCGTATGCGTTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGC  >  NC_007793/2110060‑2110245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑