Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,103,342	A→G	G110G (GGT→GGC)	SAUSA300_RS10615 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,103,342	0	A	G	100.0%	51.1 / NA	21	G110G (GGT→GGC)	SAUSA300_RS10615	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/20);  total (1/20)

TGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTAGGTTTGATAAATTTACCTGTCCCTTTTTGAACGTGTCCATTTTCAATT  >  NC_007793/2103245‑2103402                                                                                                  |                                                             tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGcc                                                            <  1:2123668/100‑1 (MQ=255) tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCC‑TTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc                                                           <  1:1150360/100‑1 (MQ=255)  gAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc                                                           <  1:903610/100‑1 (MQ=255)    aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt                                                         <  1:4269813/100‑1 (MQ=255)    aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt                                                         <  1:4113529/100‑1 (MQ=255)    aCTTTGTACAAAATATCAATCACAA‑TTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCagg                                                        <  1:1207552/100‑3 (MQ=255)     cTTTGTAAAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCtata                                                        <  1:4326796/100‑1 (MQ=255)      tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAtta                                                       <  1:4969403/100‑3 (MQ=255)      tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAg                                                       <  1:1040512/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATGCTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:4342110/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGccag                                                   <  1:2069520/100‑4 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2264974/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2023722/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:4571133/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2008558/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:5125877/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:5779018/100‑1 (MQ=255)          tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:1801623/100‑1 (MQ=255)           aCAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTa                                                  <  1:3548238/100‑1 (MQ=255)                                               tAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTAGGTTTGATAAATTTACCTGTTTCTTTTTGAACgtgt              <  1:4861090/100‑1 (MQ=255)                                                           aTACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTAGGGTTGATACATTTACCTGTTTCTTTTTGAACGTGTCCATATTCAAtt  >  1:3938082/1‑100 (MQ=255)                                                                                                  |                                                             TGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTAGGTTTGATAAATTTACCTGTCCCTTTTTGAACGTGTCCATTTTCAATT  >  NC_007793/2103245‑2103402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑