Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,103,339	G→A	G111G (GGC→GGT)	SAUSA300_RS10615 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,103,339	0	G	A	100.0%	61.3 / NA	23	G111G (GGC→GGT)	SAUSA300_RS10615	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/22);  total (1/22)

ATGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTAGGTTTGATAAATTTACCTGTCCCTTTTTGAACGTGTCCATTTTCAATT  >  NC_007793/2103244‑2103402                                                                                                |                                                                aTGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACAccgc                                                             <  1:1094936/100‑3 (MQ=255)  tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACAccgta                                                            <  1:4089179/100‑4 (MQ=255)  tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGcc                                                            <  1:2123668/100‑1 (MQ=255)  tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCC‑TTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc                                                           <  1:1150360/100‑1 (MQ=255)   gAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc                                                           <  1:903610/100‑1 (MQ=255)     aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt                                                         <  1:4269813/100‑1 (MQ=255)     aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt                                                         <  1:4113529/100‑1 (MQ=255)     aCTTTGTACAAAATATCAATCACAA‑TTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCagg                                                        <  1:1207552/100‑3 (MQ=255)      cTTTGTAAAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCtata                                                        <  1:4326796/100‑1 (MQ=255)       tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAtta                                                       <  1:4969403/100‑3 (MQ=255)       tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAg                                                       <  1:1040512/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATGCTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:4342110/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGccag                                                   <  1:2069520/100‑4 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2264974/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:1801623/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:4571133/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2023722/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:5125877/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:5779018/100‑1 (MQ=255)           tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt                                                   <  1:2008558/100‑1 (MQ=255)            aCAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTa                                                  <  1:3548238/100‑1 (MQ=255)                                                tAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTAGGTTTGATAAATTTACCTGTTTCTTTTTGAACgtgt              <  1:4861090/100‑1 (MQ=255)                                                            aTACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTAGGGTTGATACATTTACCTGTTTCTTTTTGAACGTGTCCATATTCAAtt  >  1:3938082/1‑100 (MQ=255)                                                                                                |                                                                ATGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTAGGTTTGATAAATTTACCTGTCCCTTTTTGAACGTGTCCATTTTCAATT  >  NC_007793/2103244‑2103402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑