Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,103,279	T→C	L131L (TTA→TTG)	SAUSA300_RS10615 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,103,279	0	T	C	100.0%	96.4 / NA	35	L131L (TTA→TTG)	SAUSA300_RS10615	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/35);  total (0/35)

TATTATTGATATTTACGTGCTCTCTAATAATTCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTA  >  NC_007793/2103192‑2103354                                                                                        |                                                                            tattatTGATATTTACGTGCTCTCTAATAATTCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCttttt                                                                 <  1:2952456/100‑1 (MQ=255)         atatTTACGTGCTCTCTAATAATTCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGtctc                                                         <  1:4346923/100‑1 (MQ=255)             ttACGTGCTCTCTAATAATTCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCacag                                                     <  1:3802502/100‑4 (MQ=255)                                tCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTc                                  <  1:3333988/100‑1 (MQ=255)                                  tGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCtt                                <  1:3472483/100‑1 (MQ=255)                                  tGTCTCGACTAATCACTTCATGAACTCTGTACAAAATATCAATCCCAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCtt                                <  1:5385052/100‑1 (MQ=255)                                   gTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTa                               <  1:1385035/100‑1 (MQ=255)                                     cTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTAtt                             <  1:220044/100‑1 (MQ=255)                                      ttGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTAtgc                            <  1:975297/100‑3 (MQ=255)                                         aCTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGctt                         <  1:2758328/100‑2 (MQ=255)                                             aTCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTAtt                     <  1:867905/100‑1 (MQ=255)                                             aTCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTAtt                     <  1:1052549/100‑1 (MQ=255)                                             aTCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTAtt                     <  1:4328794/100‑1 (MQ=255)                                               cACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTaa                   <  1:159858/100‑1 (MQ=255)                                                     aTGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACAccgc             <  1:1094936/100‑3 (MQ=255)                                                      tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACAccgta            <  1:4089179/100‑4 (MQ=255)                                                      tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGcc            <  1:2123668/100‑1 (MQ=255)                                                      tGAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCC‑TTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc           <  1:1150360/100‑1 (MQ=255)                                                       gAACTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGccc           <  1:903610/100‑1 (MQ=255)                                                         aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt         <  1:4113529/100‑1 (MQ=255)                                                         aCTTTGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAt         <  1:4269813/100‑1 (MQ=255)                                                         aCTTTGTACAAAATATCAATCACAA‑TTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCagg        <  1:1207552/100‑3 (MQ=255)                                                          cTTTGTAAAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCtata        <  1:4326796/100‑1 (MQ=255)                                                           tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCAtta       <  1:4969403/100‑3 (MQ=255)                                                           tttGTACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAg       <  1:1040512/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATGCTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:4342110/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGccag   <  1:2069520/100‑4 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:4571133/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:1801623/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:5125877/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:2264974/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:5779018/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:2008558/100‑1 (MQ=255)                                                               tACAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCttt   <  1:2023722/100‑1 (MQ=255)                                                                aCAAAATATCAATCACAATTTTTTCAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACACCGCCCATAGCTTTa  <  1:3548238/100‑1 (MQ=255)                                                                                        |                                                                            TATTATTGATATTTACGTGCTCTCTAATAATTCTGTCTTGACTAATCACTTCATGAACTTTGTACAAAATATCAATCACAATTTTTTTAACTCCCTTTTTAGCGTCTCAAAATACTTATTTTGCCCTTGTCTTATTGCTCTATTAACGCCACCCATAGCTTTA  >  NC_007793/2103192‑2103354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑