Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,102,665	G→A	N66N (AAC→AAT)	SAUSA300_RS10605 ←	phage tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,102,665	0	G	A	100.0%	47.8 / NA	18	N66N (AAC→AAT)	SAUSA300_RS10605	phage tail protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/17);  total (1/17)

CCGTATTCATCATACTCTTCATTGAATACTATTTTGCGAATCTCTTTAGGGAAAGCATGCATTTGTAACGAAATTTTACCTTCTCCGTCTGTGTTCCCTGATTCGATTGGACCACCATCAGCATATGCTGTTTTCAAGTCTCCTCCAGTTTCAACACCGATTTTTTGTA  >  NC_007793/2102573‑2102741                                                                                             |                                                                             ccatcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTg                                                                       <  1:988841/95‑1 (MQ=255) ccatcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTg                                                                       <  1:5138992/95‑1 (MQ=255)   atcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGTGAACGCTTGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGAt                                                                     <  1:5592636/97‑1 (MQ=255)   atcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGAt                                                                     <  1:91555/97‑1 (MQ=255)           catAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTgg                                                             <  1:4745060/100‑1 (MQ=255)           catAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTgg                                                             <  1:787360/100‑1 (MQ=255)                tctTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGaccgg                                                        <  1:2272334/100‑3 (MQ=255)                    cATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATc                                                    <  1:3268869/100‑1 (MQ=255)                     aTTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGaccgccgcgt                                                   <  1:3212929/100‑5 (MQ=255)                          aaacaaTTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCata                                              <  1:4586078/95‑1 (MQ=255)                          aaacaaTTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCata                                              <  1:5687523/95‑1 (MQ=255)                                ttttGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGt                                        <  1:4542325/100‑1 (MQ=255)                                                  ggAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGt                      <  1:5123135/100‑1 (MQ=255)                                                    aacgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGttt                    >  1:5474265/4‑100 (MQ=255)                                                     acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc                   <  1:4093226/98‑1 (MQ=255)                                                     acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc                   <  1:1374912/98‑1 (MQ=255)                                                     acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc                   <  1:3387196/98‑1 (MQ=255)                                                                      gagaTTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTCAACACCAATTTTTTGTa  <  1:3116224/97‑1 (MQ=255)                                                                                             |                                                                             CCGTATTCATCATACTCTTCATTGAATACTATTTTGCGAATCTCTTTAGGGAAAGCATGCATTTGTAACGAAATTTTACCTTCTCCGTCTGTGTTCCCTGATTCGATTGGACCACCATCAGCATATGCTGTTTTCAAGTCTCCTCCAGTTTCAACACCGATTTTTTGTA  >  NC_007793/2102573‑2102741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑