Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	48,418	T→A	E158D (GAA→GAT)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	48,418	0	T	A	100.0%	14.2 / NA	7	E158D (GAA→GAT)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/3);  total (4/3)

TCTTATAACCATGACCTTTAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATATTCGTAACCAAAAGGTGCATGTGTTGTAATCAATCGACCTTGCTTTGCTTTTTCTTGAAGCC  >  NC_007793/48327‑48479                                                                                            |                                                              tCTTATAACCATGACCTTTAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAAcc                                                       <  1:4589744/100‑1 (MQ=255)          gaTGACCTTTAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGgcgc                                              >  1:1601488/2‑100 (MQ=255)              aCCTTTAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGGCGCAtgg                                          <  1:534905/100‑2 (MQ=255)                  ttAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGGCGCATGggttg                                      <  1:2928800/100‑1 (MQ=255)                                   tACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGGCGCATGGGTTGTAATCAATCGACCttgc                     >  1:5261579/1‑100 (MQ=255)                                    aCAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGGCGCATGGGTTGTAATCAATCGACCttgct                    >  1:63969/1‑100 (MQ=255)                                                      gACTCATTTTGATTGATGATGAATGTTCCGTTGTGATAATCGTAACCAAAGGGCGCATGGGTTGTAATCAATCGACCTTGCTTTGCTTTTTCTTGAAGcc  >  1:2576986/1‑100 (MQ=255)                                                                                            |                                                              TCTTATAACCATGACCTTTAATGTAATAATTGAATACAGCCTTTACCGTTGGTGACTCATTTTGATTGATGATGAATGTTCCGTTGTGATATTCGTAACCAAAAGGTGCATGTGTTGTAATCAATCGACCTTGCTTTGCTTTTTCTTGAAGCC  >  NC_007793/48327‑48479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑