D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	21,561	G→A	V259I (GTT→ATT)	SAUSA300_RS00085 →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	21,561	0	G	A	99.2%	1989.5 / ‑2.8	707	V259I (GTT→ATT)	SAUSA300_RS00085	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (668/33); total (673/34)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 707 total aligned reads displayed.

AGTTGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATGTTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCGGTAGGTAAATTATCACGTACGAAGATTTTT  >  NC_007793/21464‑21656

  ttGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGa                                                                                             >  1:3696357/1‑100 (MQ=255)

   tGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGAc                                                                                            >  1:5190091/1‑100 (MQ=255)

   tGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGAc                                                                                            >  1:1347473/1‑100 (MQ=255)

   tGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGAc                                                                                            >  1:5563635/1‑100 (MQ=255)

    gCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACt                                                                                           >  1:3284639/1‑100 (MQ=255)

    gCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACt                                                                                           >  1:5139391/1‑100 (MQ=255)

    gCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACt                                                                                           >  1:3833700/1‑100 (MQ=255)

    gCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACt                                                                                           >  1:1502650/1‑100 (MQ=255)

     taaCGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTc                                                                                          >  1:1080735/2‑100 (MQ=255)

     cAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTc                                                                                          >  1:4989772/1‑100 (MQ=255)

      aaCGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGCTGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCa                                                                                         >  1:5642131/1‑100 (MQ=255)

      aaCGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCa                                                                                         >  1:530937/1‑100 (MQ=255)

       aCGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCaa                                                                                        >  1:2248817/1‑100 (MQ=255)

       aCGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCaa                                                                                        >  1:2869784/1‑100 (MQ=255)

        ggCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCaaa                                                                                       >  1:1286286/2‑100 (MQ=255)

        cGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCaaa                                                                                       >  1:4665170/1‑100 (MQ=255)

           aTGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCg                                                                                    >  1:3735465/1‑100 (MQ=255)

                   atGTATACAGTTGGTATTTTCTAGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGGAGTTCTGGAAATATTGACTCAAACCGCTTAAGaa                                                                            >  1:1045578/1‑100 (MQ=255)

                     gTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACg                                                                          >  1:2636667/1‑100 (MQ=255)

                                   cttgctcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGa                                                            >  1:1173467/5‑100 (MQ=255)

                                    tttCTCGCTAGCGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAg                                                           >  1:5235993/1‑100 (MQ=255)

                                    tttCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAg                                                           >  1:569793/1‑100 (MQ=255)

                                    tttCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATATGAAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAg                                                           >  1:1659918/1‑100 (MQ=255)

                                     ttCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAgg                                                          >  1:4849043/1‑100 (MQ=255)

                                     ttCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACGGAgg                                                          >  1:4484943/1‑100 (MQ=255)

                                     ttCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGCACTATGACTGAgg                                                          >  1:3650074/1‑100 (MQ=255)

                                     ggcccGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAgg                                                          >  1:3791842/5‑100 (MQ=255)

                                     ctctcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAgg                                                          >  1:1871575/2‑100 (MQ=255)

                                      ctccGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACAGAGGa                                                         >  1:5280803/4‑100 (MQ=255)

                                      ccccGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGa                                                         >  1:4117033/4‑100 (MQ=255)

                                      actcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGa                                                         >  1:5404598/2‑100 (MQ=255)

                                       ttcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGaa                                                        >  1:3022510/2‑100 (MQ=255)

                                       ctcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGaa                                                        >  1:3342676/1‑100 (MQ=255)

                                       ctcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGaa                                                        >  1:748778/1‑100 (MQ=255)

                                       ctcGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGaa                                                        >  1:3558647/1‑100 (MQ=255)

                                        ccGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAg                                                       >  1:2575695/2‑100 (MQ=255)

                                         cGCTCGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACACAAACCGCTTAAGCACGGGCACTATGACTGAGGAAGa                                                      >  1:906933/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGCTCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1686623/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTGGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:2538172/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGATCGAGGagat                                                      >  1:2763990/1‑97 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAgggaag                                                      >  1:603170/1‑96 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:961843/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:5480552/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:5081984/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:4946847/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:3964291/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:4741033/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:4021111/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:4685954/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:4259635/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1117228/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1377687/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1763163/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1805781/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:3196442/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:2059905/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:2212326/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:2282762/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:2328315/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTCAGGAAGa                                                      >  1:3420531/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACGGAGGAAGa                                                      >  1:1459564/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACGGAGGAAGa                                                      >  1:247640/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGa                                                      >  1:2141572/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGa                                                      >  1:2976942/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGa                                                      >  1:4546006/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGa                                                      >  1:4218744/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACAGAGGAAGa                                                      >  1:576771/1‑100 (MQ=255)

                                         cGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGCAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACAGAGGAAGa                                                      >  1:2394908/1‑100 (MQ=255)

                                         cGCTAGAGATGGGCGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:3394654/1‑100 (MQ=255)

                                         cGCTAGAGATGGGCGCTGATCAGTTAGCCACACGAATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGa                                                      >  1:1906021/1‑100 (MQ=255)

                                          ccTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGAt                                                     >  1:3111636/2‑100 (MQ=255)

                                           cTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGCTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGAtt                                                    >  1:1982471/1‑100 (MQ=255)

                                             ttagaTGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATCgg                                                  >  1:2416666/3‑100 (MQ=255)

                                             agagATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTgg                                                  >  1:675569/1‑100 (MQ=255)

                                             agagATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATCgg                                                  >  1:4335201/1‑100 (MQ=255)

                                             agagATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTAAGGAAGATCgg                                                  >  1:1211470/1‑100 (MQ=255)

                                             agagATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGAATGAGGagatcgga                                                  >  1:2177449/1‑93 (MQ=255)

                                               tgaTGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGATCGGaa                                                >  1:4378163/2‑99 (MQ=255)

                                               agaTGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATCGGaa                                                >  1:1250306/1‑99 (MQ=255)

                                                taTGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGATGAAGATTGGAGt                                               >  1:5357876/2‑100 (MQ=255)

                                                    ggTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACCGAGGAAGATCGGaagagca                                           >  1:390367/1‑94 (MQ=255)

                                                          gATCAGTTAGCCACACGTATGATCTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACta                                     >  1:3059615/1‑100 (MQ=255)

                                                             ctgTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAg                                  >  1:492128/3‑100 (MQ=255)

                                                             cAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAg                                  >  1:158949/1‑100 (MQ=255)

                                                                 tAGCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCgct                              <  1:1403194/100‑3 (MQ=255)

                                                                 gtgctacacGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggt                              >  1:5038848/6‑100 (MQ=255)

                                                                  tgCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggcg                             >  1:1937696/2‑98 (MQ=255)

                                                                  cgCCACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggta                             >  1:5616677/2‑100 (MQ=255)

                                                                  aaccACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggta                             >  1:3132906/3‑100 (MQ=255)

                                                                  aGCCACCCGTATGATTTGTAGTTCTGGAAATGTTGACTCAAACCGCTTAAGCACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggta                             >  1:2911229/1‑100 (MQ=255)

                                                                  aGCCACACGTATGATTTGTAGTTCTGGAGATATTGACTCAAACCGCTTAAGAACGGGTACTATGGCTGAGGAAGATTGGAGTCGTTTTACTATAGCggta                             >  1:4425470/1‑100 (MQ=255)

                                                                   gtgacacGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtag                            >  1:3589937/4‑100 (MQ=255)

                                                                   gacacaCGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtag                            >  1:5525713/3‑100 (MQ=255)

                                                                    ccACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtagg                           >  1:2814539/1‑100 (MQ=255)

                                                                    ccACACGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtagg                           >  1:1001410/1‑100 (MQ=255)

                                                                     cacaCGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtaggt                          >  1:3240176/1‑100 (MQ=255)

                                                                      acacGTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCggtaggta                         >  1:2714078/1‑100 (MQ=255)

                                                                         agTATGATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGACGTTTTACTATAGCGGTAGGTAAAt                      >  1:3504554/2‑100 (MQ=255)

                                                                              gATTTGTAGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCGGTAGGTAAATTATCa                 >  1:4632300/1‑100 (MQ=255)

                                                                                     aGTTCTGGAAATATTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCGGTAGGTAAATTATCACGTACGa          >  1:2509848/1‑100 (MQ=255)

AGTTGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCGCTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGAAATGTTGACTCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCGGTAGGTAAATTATCACGTACGAAGATTTTT  >  NC_007793/21464‑21656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`