Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,102,644	A→G	I73I (ATT→ATC)	SAUSA300_RS10605 ←	phage tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,102,644	0	A	G	100.0%	49.9 / NA	20	I73I (ATT→ATC)	SAUSA300_RS10605	phage tail protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/19);  total (1/19)

TATAAACACCGTATTCATCATACTCTTCATTGAATACTATTTTGCGAATCTCTTTAGGGAAAGCATGCATTTGTAACGAAATTTTACCTTCTCCGTCTGTGTTCCCTGATTCGATTGGACCACCATCAGCATATGCTGTTTTCAAGTCTCCTCCAGTTTC  >  NC_007793/2102565‑2102724                                                                                |                                                                                 cgtaAACGCCATCTTCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCtgt                                                              <  1:2996373/98‑1 (MQ=255) cgtaAACGCCATCTTCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCtgt                                                              <  1:3866519/98‑1 (MQ=255)   taAACGCCATCTTCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCtgtat                                                            <  1:1247571/100‑3 (MQ=255)         ccatcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTg                                                      <  1:988841/95‑1 (MQ=255)         ccatcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTg                                                      <  1:5138992/95‑1 (MQ=255)           atcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGTGAACGCTTGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGAt                                                    <  1:5592636/97‑1 (MQ=255)           atcttCATCATAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGAt                                                    <  1:91555/97‑1 (MQ=255)                   catAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTgg                                            <  1:787360/100‑1 (MQ=255)                   catAATCTTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTgg                                            <  1:4745060/100‑1 (MQ=255)                        tctTCATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGaccgg                                       <  1:2272334/100‑3 (MQ=255)                            cATTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATc                                   <  1:3268869/100‑1 (MQ=255)                             aTTAAAAACAATTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGaccgccgcgt                                  <  1:3212929/100‑5 (MQ=255)                                  aaacaaTTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCata                             <  1:4586078/95‑1 (MQ=255)                                  aaacaaTTTTGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCata                             <  1:5687523/95‑1 (MQ=255)                                        ttttGCGAATCTCTTTAGGGAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGt                       <  1:4542325/100‑1 (MQ=255)                                                          ggAACGCATGCATTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGt     <  1:5123135/100‑1 (MQ=255)                                                            aacgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGttt   >  1:5474265/4‑100 (MQ=255)                                                             acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc  <  1:4093226/98‑1 (MQ=255)                                                             acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc  <  1:3387196/98‑1 (MQ=255)                                                             acgcatgcatTTGTAATGAGATTTTACCTTCTCCGTCTGTATTCCCTGATTCAATTGGACCGCCATCAGCATAAGCTGTTTTTAGTTCTCCACCAGTTTc  <  1:1374912/98‑1 (MQ=255)                                                                                |                                                                                 TATAAACACCGTATTCATCATACTCTTCATTGAATACTATTTTGCGAATCTCTTTAGGGAAAGCATGCATTTGTAACGAAATTTTACCTTCTCCGTCTGTGTTCCCTGATTCGATTGGACCACCATCAGCATATGCTGTTTTCAAGTCTCCTCCAGTTTC  >  NC_007793/2102565‑2102724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑