Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,102,500	G→A	G121G (GGC→GGT)	SAUSA300_RS10605 ←	phage tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,102,500	0	G	A	93.3%	33.0 / ‑4.5	15	G121G (GGC→GGT)	SAUSA300_RS10605	phage tail protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (4/10);  total (4/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

AATCCCAATCTTTCTCAGCCGTTTCGCCATCGATTTTAGGATTTGTAAACATGACTTTAGGTAATAAAACCGTTCTAAATGTGCCGTCTCGACGCTCTTGTCTGAACCAAATTGCTACATAGT  >  NC_007793/2102418‑2102540                                                                                   |                                         taTCCCAATCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTg                         >  1:1117736/2‑100 (MQ=255)    tccgaTCTTTCTCGGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCt                      <  1:1889855/96‑1 (MQ=255)    ctcttccgatctcAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCt                      <  1:5039288/91‑1 (MQ=255)    cccAATCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCt                      <  1:3845599/100‑1 (MQ=255)      cAATCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTGCCGTCTCTACGCTCTTGTCTGa                    <  1:875877/100‑1 (MQ=255)      cAATCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGa                    <  1:5321954/100‑1 (MQ=255)       gaTCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTgctgtca                   <  1:5379014/99‑7 (MQ=255)         tCTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAAcc                 <  1:1685116/100‑1 (MQ=255)          cTTTCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCa                <  1:3643284/100‑1 (MQ=255)           tttCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCat               <  1:3482160/100‑2 (MQ=255)            ttCTCAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCata              >  1:1958572/1‑98 (MQ=255)               acAGCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCatacag           >  1:5713592/2‑95 (MQ=255)                  gCCGTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCatacaggag        <  1:3144461/100‑9 (MQ=255)                     gTTTCTCCATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTCTGAACCATACAGCTAcgt     <  1:3318101/100‑3 (MQ=255)                        ttcccATCGATTTTAGGATTTGTAAACATAACTTTAGGTAATAAAACTGTTCTAAATGTACCGTCTCTACGCTCTTGTATGAACCATACAGCTACGTaat  >  1:3937633/4‑98 (MQ=255)                                                                                   |                                         AATCCCAATCTTTCTCAGCCGTTTCGCCATCGATTTTAGGATTTGTAAACATGACTTTAGGTAATAAAACCGTTCTAAATGTGCCGTCTCGACGCTCTTGTCTGAACCAAATTGCTACATAGT  >  NC_007793/2102418‑2102540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑