Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	48,123	G→A	P257S (CCT→TCT)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	48,123	0	G	A	100.0%	11.8 / NA	6	P257S (CCT→TCT)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

TCTTTTTTCTAATGGTCATATTTGTAAGTGTTGCATTACAACATGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGGTGTCTGTTTGGTTTGTTTTTGCAATCGAAGTCTCTGCGCTTGCTCATATATACTTGTGGAAACAATAGAAGGAAACATATTGTCGAATTGGCCATA  >  NC_007793/48042‑48219                                                                                  |                                                                                                 cttccgatCTAATGGTCATATTTGTAAGTGTTGCATTACAACATGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGATGTCTGTTTGgtttgttt                                                                                <  1:3218323/93‑1 (MQ=255)          tAATGGTCATATTTGTAAGTGTTGCATTACAACATGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGATGTCTGTTTGGTTTGTTTTTGCAAtta                                                                       <  1:4500583/100‑3 (MQ=255)           aaTGGTCATATTTGTAAGTGTTGCATTACAACATGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGATGTCTGTTTGGTTTGTTTTTGCAATCGa                                                                      >  1:383434/1‑100 (MQ=255)                                           aTGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGATGTCTGTTTGGTTTGTTTTTGCAATCGAAGTCTCTGCGCTTGCTCATATATACTTGTGGa                                      >  1:748692/1‑100 (MQ=255)                                             gggCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGATGTCTGTTTGGTTTGTTTTTGCAATCGAAGTCTCTGCGCTTGCTCATATATACTTGTGGaaa                                    >  1:462448/1‑100 (MQ=255)                                                                               aaGATGTCTGTTTGGTTTGTTTTTGCAATCGAAGTCTCTGCGCTTGCTCATATATACTTGTGGAAACAATAGAAGGAAACATATTGTCGAATTGACCata  >  1:3830279/1‑100 (MQ=255)                                                                                  |                                                                                                 TCTTTTTTCTAATGGTCATATTTGTAAGTGTTGCATTACAACATGGGCATTTGATTTTTTGTTTGAGTTGATTATCCGAAGGTGTCTGTTTGGTTTGTTTTTGCAATCGAAGTCTCTGCGCTTGCTCATATATACTTGTGGAAACAATAGAAGGAAACATATTGTCGAATTGGCCATA  >  NC_007793/48042‑48219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑