Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,102,215	G→T	intergenic (‑313/+3)	SAUSA300_RS10600 ← / ← SAUSA300_RS10605	hypothetical protein/phage tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,102,215	0	G	T	100.0%	25.1 / NA	10	intergenic (‑313/+3)	SAUSA300_RS10600/SAUSA300_RS10605	hypothetical protein/phage tail protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/7);  total (3/7)

TGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATTTTCTTTAAGAAAGC  >  NC_007793/2102138‑2102289                                                                              |                                                                           tgtgTCGCCAACTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTcttcgtact                                                      <  1:4923419/100‑9 (MQ=255)         cAACTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTc                                              <  1:2693782/100‑1 (MQ=255)           tcTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCAc                                            <  1:4007041/99‑1 (MQ=255)           aCTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTTAc                                            <  1:4311062/100‑1 (MQ=255)           aCTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCAc                                            <  1:1666368/100‑1 (MQ=255)               taaGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCACGttg                                        <  1:624/98‑2 (MQ=255)                           tgcTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCACGTTTCCAGTATAttct                            >  1:5759861/3‑100 (MQ=255)                                           aaCCGACTTTACCGCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATTTTCt            <  1:3625245/100‑1 (MQ=255)                                                    taccgcAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATTTTCTTTAAGAAAg   >  1:3371061/6‑100 (MQ=255)                                                     gccgcAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCTTCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATTTTCTTTAAGAAAGc  >  1:22247/5‑100 (MQ=255)                                                                              |                                                                           TGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATTTTCTTTAAGAAAGC  >  NC_007793/2102138‑2102289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑