Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,886	T→C	S336G (AGT→GGT)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,886	0	T	C	100.0%	35.2 / NA	15	S336G (AGT→GGT)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/9);  total (6/9)

GTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATGTGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACTTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGCACACTTCTAATACTTTAT  >  NC_007793/47799‑47948                                                                                        |                                                               ccgCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTtgt                                                    >  1:3434926/3‑100 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCTCCTTTAATTTTTgtgt                                                  <  1:4014414/100‑1 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  <  1:1671111/100‑1 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  >  1:2073937/1‑100 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  <  1:3231125/100‑1 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  <  1:3350371/100‑1 (MQ=255)   gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  <  1:4752805/100‑1 (MQ=255)   ctcttccgatCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                                                  <  1:1959358/91‑1 (MQ=255)             atctatcttgttCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTg                                        <  1:2523546/93‑1 (MQ=255)                              gTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGtctc                       >  1:482217/1‑100 (MQ=255)                                 gatctgtgATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGc                    <  1:1960975/96‑1 (MQ=255)                                    atgtgATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGcaca                 <  1:3752029/99‑1 (MQ=255)                                              cttCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGCACACTTCTAATAc       >  1:4467253/2‑100 (MQ=255)                                              cttCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGCACACTTCTAATAc       >  1:537037/2‑100 (MQ=255)                                                   aTGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGCACACTTCTAATACTTTAt  >  1:5046072/1‑100 (MQ=255)                                                                                        |                                                               GTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATGTGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACTTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTCGGCACACTTCTAATACTTTAT  >  NC_007793/47799‑47948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑