Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,101,680	C→T	A75T (GCA→ACA)	SAUSA300_RS10600 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,101,680	0	C	T	100.0%	19.6 / NA	9	A75T (GCA→ACA)	SAUSA300_RS10600	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/9);  total (0/9)

GAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAGGTCTTTAACTGCGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGCTTCATCGTGCTATTTTCGTCCTCAATATCAT  >  NC_007793/2101626‑2101772                                                       |                                                                                             gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                                                 <  1:2823113/100‑1 (MQ=255) gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                                                 <  1:3521161/100‑1 (MQ=255) gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                                                 <  1:4817403/100‑1 (MQ=255)     tGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCg                                             <  1:2564666/100‑1 (MQ=255)                      gtgcgtgcATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATACATCAATCTGTCAGCGATTTCTCTTGGTTTCAt                            <  1:3426267/100‑1 (MQ=255)                           tgcatACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTACTCTTGGTTTCATCGTGc                       <  1:433811/100‑1 (MQ=255)                            gcatACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGTTTCATCGTGCt                      <  1:5179310/100‑1 (MQ=255)                             catACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCTATCAATCTGTCAGCGATTTCTCTTGGTTTCATCGTGCTa                     <  1:1657918/100‑1 (MQ=255)                                                ttAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGTTTCATCGTGCTATTTTCGTCCTCAATatcat  <  1:2111999/100‑1 (MQ=255)                                                       |                                                                                             GAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAGGTCTTTAACTGCGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGCTTCATCGTGCTATTTTCGTCCTCAATATCAT  >  NC_007793/2101626‑2101772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑