Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,101,668	G→A	L79L (CTA→TTA)	SAUSA300_RS10600 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,101,668	0	G	A	100.0%	21.6 / NA	9	L79L (CTA→TTA)	SAUSA300_RS10600	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/9);  total (0/9)

TTCCTCAGTTTGTTGACCTTGCGTAATGAAAACCACTTGTTCACGAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAGGTCTTTAACTGCGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGCTTCATCGTGCTA  >  NC_007793/2101582‑2101753                                                                                       |                                                                                      ttCCTCAGTTTGCTGACCTTGAGTAATGAAAATTACTTGTTCACGAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTgtg                                                                          <  1:1407585/100‑3 (MQ=255)                                             gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                              <  1:2823113/100‑1 (MQ=255)                                             gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                              <  1:3521161/100‑1 (MQ=255)                                             gAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTc                              <  1:4817403/100‑1 (MQ=255)                                                 tGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCg                          <  1:2564666/100‑1 (MQ=255)                                                                  gtgcgtgcATACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATACATCAATCTGTCAGCGATTTCTCTTGGTTTCAt         <  1:3426267/100‑1 (MQ=255)                                                                       tgcatACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTACTCTTGGTTTCATCGTGc    <  1:433811/100‑1 (MQ=255)                                                                        gcatACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGTTTCATCGTGCt   <  1:5179310/100‑1 (MQ=255)                                                                         catACGTTCTTTTAAGTCTTTAACTGTGAATTGGTTATCGTAAATTTTTACAACCATATCTATCAATCTGTCAGCGATTTCTCTTGGTTTCATCGTGCTa  <  1:1657918/100‑1 (MQ=255)                                                                                       |                                                                                      TTCCTCAGTTTGTTGACCTTGCGTAATGAAAACCACTTGTTCACGAAGTGCATTCATTCCATCAGGTGCATGCATACGTTCTTTTAGGTCTTTAACTGCGAATTGGTTATCGTAAATTTTTACAACCATATCCATCAATCTGTCAGCGATTTCTCTTGGCTTCATCGTGCTA  >  NC_007793/2101582‑2101753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑