Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,101,111	G→A	T66I (ACA→ATA)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,101,111	0	G	A	100.0%	11.7 / NA	6	T66I (ACA→ATA)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

GTCGTAACTTTTGAGCTCCTGCACTGTTTTCGCCCTGTTCTTGAGATACCTTGTCATATTGCTTAGCTAAATCATCAACGTTTTTCTTATAACCTGTGATAGTTCCATCAAGTTCTTTAATCCTTTGTTTGTAACTATCAGTTGATTTTTCGGTATATTTGAAGTTGTTGCCTGTT  >  NC_007793/2101016‑2101191                                                                                                |                                                                                 gccGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAATCATCAACATTTTTCTTATAACCTATGAt                                                                              >  1:3468290/3‑100 (MQ=255) gccGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAATCATCAACATTTTTCTTATAACCTATGAt                                                                              <  1:441957/98‑1 (MQ=255)                   cTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAATCATCAACTTTTTTCTTATAACCTATGATAGTTCCGTCAAGTTCttt                                                            >  1:2211669/1‑100 (MQ=255)                                 cccTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAATCATCAACATTTTTCTTATAACCTATGATAGTTCCGTCAAGTTCTTTAATCCTttgttggt                                              <  1:3981476/100‑1 (MQ=255)                                                                         catcaACATTTTTCTTATAACCTATGATAGTTCCGTCAAGTTCTTTAATCCTTTGTTGGTAACTATCAGTTGATTTTTCGGTATATTTGAAGTTGTTGcc      <  1:4833247/100‑1 (MQ=255)                                                                             aaCATTTTTCTTATAACCTATGATAGTTCCGTCAAGTTCTTTAATCCTTTGTTGGTAACTATCAGTTGATTTTTCGGTATATTTGAAGTTGTTGCCGGtt  <  1:5470751/100‑1 (MQ=255)                                                                                                |                                                                                 GTCGTAACTTTTGAGCTCCTGCACTGTTTTCGCCCTGTTCTTGAGATACCTTGTCATATTGCTTAGCTAAATCATCAACGTTTTTCTTATAACCTGTGATAGTTCCATCAAGTTCTTTAATCCTTTGTTTGTAACTATCAGTTGATTTTTCGGTATATTTGAAGTTGTTGCCTGTT  >  NC_007793/2101016‑2101191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑