Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,834	G→A	T353I (ACA→ATA)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,834	0	G	A	100.0%	39.2 / NA	15	T353I (ACA→ATA)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/11);  total (4/11)

GCGGTTGTTGACGTAATGATTGTGTTTGCTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATGTGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACTTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTC  >  NC_007793/47739‑47927                                                                                                |                                                                                              gCGGTTGTTGACGTAATGATTGCGTTTGTTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAAtatgtg                                                                                           <  1:4716070/100‑1 (MQ=255)                            gttctctGAACGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCttag                                                                <  1:656381/98‑3 (MQ=255)                                   gAACGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGt                                                         >  1:1207451/1‑100 (MQ=255)                                    aaCGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGtt                                                        <  1:3177399/100‑1 (MQ=255)                                        tttCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGta                                                    <  1:3830321/100‑2 (MQ=255)                                        tttCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTc                                                    <  1:3143715/100‑1 (MQ=255)                                                             ccgCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTtgt                               >  1:3434926/3‑100 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCTCCTTTAATTTTTgtgt                             <  1:4014414/100‑1 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             <  1:1671111/100‑1 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             >  1:2073937/1‑100 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             <  1:3231125/100‑1 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             <  1:3350371/100‑1 (MQ=255)                                                               gCCAACTTTTCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             <  1:4752805/100‑1 (MQ=255)                                                               ctcttccgatCTATCAGTTGTTCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTgtgt                             <  1:1959358/91‑1 (MQ=255)                                                                         atctatcttgttCTTGAGTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTg                   <  1:2523546/93‑1 (MQ=255)                                                                                          gTCAATATGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACCTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGtctc  >  1:482217/1‑100 (MQ=255)                                                                                                |                                                                                              GCGGTTGTTGACGTAATGATTGTGTTTGCTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCAACTTTTCTATCAGTTGTTCTTGAGTCAATGTGTGATGTTTTTCTATGTTTCTTTGTCTTTTGATGCGTTTGTCAATCGCACTTTTAATTTTTGTGTAGATGCGTTGATTTTGATAAAAGTCTC  >  NC_007793/47739‑47927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑