Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,101,017	T→C	R97R (CGA→CGG)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,101,017	0	T	C	100.0%	27.1 / NA	12	R97R (CGA→CGG)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/12);  total (0/12)

TGATGTTTTTTGTAATTCTCTTTCTAAATAATTCAGTTCATTTGCTTGTTTGTTATATTCTTGTCGTAACTTTTGAGCTCCTGCACTGTTTTCGCCCTGTTCTTGAGATACCTTGTCATATTGCTTAGCTAAATCATCAACGTTTTTCTTATAACCTG  >  NC_007793/2100954‑2101111                                                                |                                                                                               cgATGTTTTTTGCAATTCTCTTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCCGCACTGTTTTCAcccgcg                                                            <  1:3886189/99‑4 (MQ=255)      ttttttGCAATTCTCTTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTg                                                       <  1:1582376/100‑1 (MQ=255)      ttttttGCAATTCTCTTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTg                                                       <  1:547994/100‑1 (MQ=255)       tttttGCAATTCTCTTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTgg                                                      <  1:3229010/100‑2 (MQ=255)                  ctctTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTc                                           <  1:5617698/100‑1 (MQ=255)                  ctctTTCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTc                                           <  1:5707703/100‑1 (MQ=255)                       tCTAAATAATTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATAtt                                      <  1:5402591/100‑1 (MQ=255)                               atTCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCt                              <  1:4419539/100‑1 (MQ=255)                                 tCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTaa                            <  1:2231766/100‑1 (MQ=255)                                 tCAGCTCATTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTaa                            <  1:66503/100‑1 (MQ=255)                                        aTTTGCTTGTTTGTTATATTCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAAtcatca                     <  1:729503/100‑1 (MQ=255)                                                           tCTTGCCGTAATTTTTGAGCTTCTGCACTGTTTTCACCCTGTTCTTGAGATACCTTGTCATATTGCTTGGCTAAATCATCAACATTTTTCTTATAACCta  <  1:402736/100‑2 (MQ=255)                                                                |                                                                                               TGATGTTTTTTGTAATTCTCTTTCTAAATAATTCAGTTCATTTGCTTGTTTGTTATATTCTTGTCGTAACTTTTGAGCTCCTGCACTGTTTTCGCCCTGTTCTTGAGATACCTTGTCATATTGCTTAGCTAAATCATCAACGTTTTTCTTATAACCTG  >  NC_007793/2100954‑2101111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑