Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,100,948	T→C	E120E (GAA→GAG)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,100,948	0	T	C	100.0%	13.7 / NA	7	E120E (GAA→GAG)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/2);  total (5/2)

TAGGTCCCATACTTTCAAAAACTTTACTGGTTTTTCCCCAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGAGCTTTTTTGAACTCTTCAAATTCGGCTGATGTTTTTTGTAATTCTCTTTCTAAATAATTCAGTTCATTTGCTTGTTTG  >  NC_007793/2100854‑2101005                                                                                               |                                                          tAGGTCCCATACTTTCAAAAATTTTACTGGTTTTCCCCCAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGc                                                      >  1:1073842/1‑100 (MQ=255)                          aCTGGTTTTCCCCCAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGTAATTCTCTTTCt                             >  1:5381091/1‑100 (MQ=255)                                   tCCCCAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGCAATTCTCTTTCTAAATAATTc                    >  1:1420593/1‑100 (MQ=255)                                      tcAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGCAATTCTCTTTCTAAATAATTCAgc                 >  1:4003543/2‑99 (MQ=255)                                      ccAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGCAATTCTCTTTCTAAATAATTCAgc                 <  1:4964234/100‑2 (MQ=255)                                          ccACTTTCTGCCATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGCAATTCTCTTTCTAAATAATTCAGCTCag             <  1:2933117/100‑2 (MQ=255)                                                     cATTCTTTGAGCTTCAACTTGGGCTTTTTTGAACTCTTCAAACTCAGCCGATGTTTTTTGCAATTCTCTTTCTAAATAATTCAGCTCATTTGCttgtttg  >  1:5436442/1‑100 (MQ=255)                                                                                               |                                                          TAGGTCCCATACTTTCAAAAACTTTACTGGTTTTTCCCCAGCCACTTTCTGCCATTCTTTGAGCTTCAACTTGAGCTTTTTTGAACTCTTCAAATTCGGCTGATGTTTTTTGTAATTCTCTTTCTAAATAATTCAGTTCATTTGCTTGTTTG  >  NC_007793/2100854‑2101005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑