Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,100,795	T→A	V171V (GTA→GTT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,100,795	0	T	A	100.0%	12.4 / NA	6	V171V (GTA→GTT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/6);  total (0/6)

AGTAACAGTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCGATACCTAAAACAGGTGCAGTTACACCAATCATCATGCCTTTACCAATGGATTTTAAACCATCACCCATTTTTGTTAATTTAGGTCCCAT  >  NC_007793/2100717‑2100863                                                                               |                                                                     ggTAACTGTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCAATACCTAAAACAGGTGCGGTAACACCAATCATCATACCtttt                                                 <  1:1121698/99‑2 (MQ=255)    aaCTGTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCAATACCTAAAACAGGTGCGGTAACACCAATCATCATACCTTTACCa                                              <  1:2965453/100‑1 (MQ=255)    aaCTGTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCAATACCTAAAACAGGTGCGGTAACACCAATCATCATACCTTTACCa                                              <  1:3230664/100‑1 (MQ=255)      ctgTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCAATTCCTAAAACAGGTGCGGTAACACCAATCATCATACCTTTACCAAt                                            <  1:2632941/98‑1 (MQ=255)                     tATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCAATACCTAAAACAGGTGCGGTAACACCAATCATCATACCTTTACCAATAGATTTTAAACCATc                             <  1:5761640/100‑1 (MQ=255)                                                gatctgctgcTATACCTAAAACAGGTGCGGTAACACCAATCATCATACCTTTACCAATAGATTTTAAACCATCACCCATTTTTGTTAATTTAGGTCCCAt  <  1:3034820/97‑1 (MQ=255)                                                                               |                                                                     AGTAACAGTATCTAAACCTTTATCAACTTCTGCAAAAGCTTTTCCTGATGCTGCTGCGATACCTAAAACAGGTGCAGTTACACCAATCATCATGCCTTTACCAATGGATTTTAAACCATCACCCATTTTTGTTAATTTAGGTCCCAT  >  NC_007793/2100717‑2100863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑