Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,100,565	T→C	N248S (AAT→AGT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,100,565	0	T	C	100.0%	13.0 / NA	7	N248S (AAT→AGT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/4);  total (3/4)

CCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAGCATCTGCTGGAAAATTGCCAT  >  NC_007793/2100482‑2100655                                                                                    |                                                                                           ccATTGCACGCGTAATTAATTGAACGGCTTGTACGCCTTCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGcc                                                                            <  1:2939674/100‑1 (MQ=255)     tGCACGCGTAATTAATTGAACGGCTTGTACGCCTTCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGtg                                                                        >  1:5082703/1‑99 (MQ=255)                                   gccttCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTaaa                                          <  1:1465880/96‑1 (MQ=255)                                        cAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACAcc                                     >  1:5734136/1‑100 (MQ=255)                                            aCCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACAccgcct                                 <  1:4886600/100‑2 (MQ=255)                                                       gACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACACCGCCTACAGTCTCAgg                      <  1:3756826/100‑2 (MQ=255)                                                                           cctGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACACCGCCTACAGTCTCAGCATCTGCTGGAAAGTTGCCAt  >  1:3164217/2‑100 (MQ=255)                                                                                    |                                                                                           CCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAGCATCTGCTGGAAAATTGCCAT  >  NC_007793/2100482‑2100655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑